Highly-branched Poly(N-isopropylacrylamides) in Protein binding Studies
Post Doctoral Research Fellow working with Dr. S Rimmer, funded by the EPSRC [2004-2009]. Returned to group September 2018 – present.
Highly-branched poly-(N-isopropylacrylamides) can be formed via the copolymerisation of NIPAM with a suitable polymerisable RAFT agent such as 4-vinylbenzyl N-pyrrole dithioate. The polymers can be chain-end functionalised with peptides by first converting the chain-ends to carboxylic acids, which can be activated to succinimide and then coupled to RGD-peptides. We are exploring the capacity of these polymers to bind to integrin proteins using the techniques of gel-shift assay (EMSA), surface plasmon resonance (SPR) and Quartz Crystal Microbalance (QCM) techniques.