An advanced proof of our latest work has just gone live at Science Direct where it can be read in advance of final corrections. This work, carried out by Thomas Swift, Richard Hoskins, Richard Telford, David Pownell, Richard Plenderleith and Stephen Rimmer outlines a dual approach to preparing brand new chromatography techniques for characterising polymers.
Size Exclusion Chromatography is traditionally carried out in either aqueous or non-polar solvents. A system to present molar mass distributions of polymers using methanol as a mobile phase is presented. This is shown to be a suitable system for determining the molar mass distributions poly(N-isopropylacrylamide)s (PNIPAM); a polymer class that is often difficult to analyze by size exclusion chromatography. DOSY NMR was used to provide intrinsic viscosity data that was used in conjunction with a viscometric detector to provide absolute calibration. Then the utility of the system was shown by providing the absolute molar mass distributions of dispersed highly branched PNIPAM with biologically functional end groups.