Thermo-responsive, peptide-functional materials for cell-culture
PhD project supervised by Dr. S Rimmer and Prof. P.V. Hatton, and funded by the EPSRC [2005-2008].
The project involves synthesis of novel peptide-functional monomers and subsequent co-polymerisation to give thermo-responsive polymers which are potentially useful as cell-culture materials.
Short peptide-sequences are produced using solid-phase peptide synthesis and incorporated into polymers with controlled molecular weight and architecture, afforded by RAFT polymerisation.
Above: Polymerisation scheme for a peptide functionalised polymer; work sponsored by the EPSRC.
Publications by James Lapworth
- Thermally reversible colloidal gels for three-dimensional chondrocyte culture, Journal of the Royal Society Interface, 2012, 9, 362-375
- Thermally responsive gels formed from highly branched poly(N-isopropyl acrylamide)s with either carboxylic acid or trihistidine end groups, RSC Advances, 2013, 18108-18114
- pH Responsive Highly Branched Poly(N-isopropylacrylamide) with Trihistidine or Acid Chain Ends, RSC Advances, 2016, Accepted Manuscript
- Lapworth, J.W. Peptide-functional poly(N-isopropylacrylamides) – Poster at Macro 2006 (Warwick, UK)