A paper has been published in macromolecules bioscience which demonstrates the use of highly branched poly(N-isopropylacrlyamide) polymers for protein purification prcesses dependent on the LCST of the polymer.
Highly branched poly(NIPAM) have been prepared using the technique of reversible addition-fragmentation chain transfer (RAFT) polymerisation using a chain transfer agent that allows the incorporation of imidazole functionality in the polymer chain-ends. The lower critical solution temperature (LCST) of the polymers can be controlled by the amount of hydrophobe and GMA incorporated during copolymerisation procedures. These thermally responsive “smart” polymers were used to purify a His-tagged BRCA-1 protein fragment by affinity precipitation.
The full paper can be found here: http://dx.doi.org/10.1002/mabi.200400218